ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.
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It is for this reason that the use of the term leaching throughout this document is limited to only the testing conditions described herein. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.
Work Item s – proposed revisions of this standard. Presence of residual antimicrobial activity indicates the presence of a leaching type of antimicrobial agent. Note 1 — Stresses may include laundry, wear and abrasion, radiation and steam sterilization, UV exposure, solvent manipulation, temperature susceptibility, or similar physical or chemical manipulation.
This dynamic shake flash test was developed for routine quality control and screening test. Link to Active This link will always route to the current Active version of the standard. This method is generally used to substantiate antimicrobial properties of treated specimens for non-public health related claims.
Microbial concentrations in the treated and untreated samples are usually determined at 0, 1 or 24 hours contact time. The number of viable organisms from the suspension is determined and the percent reduction is calculated by comparing retrievals from appropriate controls. One-hour contact time in a buffer solution allows for metabolic stasis in the population.
This test method is designed to evaluate the antimicrobial activity of non-leaching, antimicrobial-treated specimens under dynamic contact e21499.
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The test quantitatively evaluates the effectiveness of a sample treated with a non-leaching antimicrobial agent by shaking in an organism suspension. We use this information to improve and customize your browsing experience and for analytics and metrics asgm our visitors — both on this website and other media. If an antimicrobial agent that is shown to be removed from the surface by Section 13 is utilized in this test methodology, controls must be included such that appropriate neutralization steps are including during recovery and enumeration.
This test method ensures good contact between the bacteria and the treated fiber, fabric, or other substrate, by constant agitation of the atm specimen in a challenge suspension during the test period. The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for the test flask as compared to the untreated control suspension.
ASTM E – Microbe Investigations (MIS)
In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli. This standard does not purport to address all of the safety concerns, if any, associated with its use.
These cookies collect information about how you interact with our website and allow us to remember you. These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility. A single cookie will be used in your browser to remember your preference not to be tracked.
ASTM E2149 Antimicrobial Activity
This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
Link to E21149 This link will always route to the current Active version of the standard.
Active view current version of standard. Materials evaluated by this method may be comprised of fabric, paper, powder or other solid materials.
This website stores cookies on your computer. Following exposure, a sample of the test organism suspension is removed quantitatively assayed for survivors.
Record and report presence of solution activity. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.
All the samples are shaken in dynamic shake flasks in a wrist action shaker to ensure good contact between the bacteria and the treated fibre, fabric, or other substrate by constant agitation of the test specimen in a bacterial suspension during the test period. No regulated reduction limits currently exist for general antimicrobial claims made using this method. Maria Curry ext Wrist Action Shaker.