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ASTM E2149 PDF

ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.

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It has been developed for routine quality control and screening tests and is used to evaluate substrate bound antimicrobials.

ASTM E2149 Antimicrobial Activity

The test determines the antimicrobial activity of a treated specimen by shaking samples of surface-bound materials in a concentrated bacterial suspension for a one hour contact time. These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility.

The test quantitatively evaluates the effectiveness of a sample treated with a non-leaching antimicrobial agent by shaking in an organism suspension. Summary of Test In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli.

Work Item s – proposed revisions of this standard. No other units of measurement are included in this standard. Materials evaluated by this method may aetm comprised of fabric, paper, powder or other solid materials. To determine if a compound is immobilized in all conditions or during the end use of the product additional testing may be required.

Note 1 — Stresses may include laundry, wear and e219, radiation and steam sterilization, UV exposure, solvent manipulation, temperature susceptibility, or similar physical or chemical manipulation. Presence of residual antimicrobial activity indicates the presence of a leaching type of antimicrobial agent.

Referenced Documents purchase separately Zstm documents listed below are referenced within the subject standard but are not provided as part of the standard. This test method ensures good contact between the bacteria and the treated fiber, fabric, or other substrate, by constant agitation of the test specimen in a challenge suspension during the test period.

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The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for the test flask as compared to the untreated control suspension. This test ensures good contact between bacteria and the treated fibre by constant agitation of the test specimen in a bacterial suspension during the test period 1hour. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility.

This website stores cookies on your computer. Link to Active This link will always route to the current Active version of the standard. e21499

ASTM E – Method Determining the Antimicrobial Activity

Maria Curry ext Wrist Action Shaker. If an antimicrobial agent that is shown to be removed from the surface by Section 13 is utilized in this test methodology, controls must be included such that appropriate neutralization steps are including during recovery and enumeration. Summary of Test Method: This test method astk both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism.

This standard does not purport to address all of the safety concerns, if any, associated with its use. This method is generally used to substantiate antimicrobial properties of treated specimens for non-public health related claims.

Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard.

These cookies collect information about how you interact with our website and allow us to remember you. Log in Log out Edit. This test method is only intended to determine efficacy as described in 4.

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This test method is designed to evaluate the awtm of specimens treated with a non-leaching antimicrobial agent to the growth of microbes under dynamic contact conditions.

No regulated reduction limits currently exist for general antimicrobial claims made using asttm method. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials. We use this information to improve and customize your browsing experience and for analytics and metrics about our visitors — both on this website and other media.

This test method is designed to evaluate the antimicrobial activity of non-leaching, antimicrobial-treated specimens under dynamic contact conditions.

The antimicrobial activity of a substrate-bound, non-leaching antimicrobial agent is dependent upon direct contact of microbes with the active chemical agent.

One-hour contact time in a buffer solution allows for metabolic stasis in the population. It is for this reason that the use of the term leaching throughout this document is limited to only the testing conditions described herein. Immobilized antimicrobial agents, such as surface bonded materials, are not free to diffuse into their environment under normal conditions of use.

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The ASTM E Standard Test Method is used to evaluate the antimicrobial activity of substrate-bound, non-leaching, antimicrobial-treated samples under dynamic contact e219. Link to Active This link will always route to the current Active version of the standard. This standard does not purport to address all of the safety concerns, if any, associated with its use.